CD133(鈭? Cells, Derived From a Single Human Colon Cancer Cell Line, Are More Resistant to 5-Fluorouracil (FU) Than CD133(+) Cells, Dependent on the 尾1-Integrin Signaling

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• 作者：Kumiko Hongo ; M.D.^&lowast ; ; ² ; ^{kimikh@attglobal.net} ; Junichiro Tanaka ; M.D. ; Ph.D.^&lowast ; ; ² ; Nelson H. Tsuno ; M.D. ; Ph.D.^&lowast ; ; ^&dagger ; ; Kazushige Kawai ; M.D. ; Ph.D.^&lowast ; ; Takeshi Nishikawa ; M.D. ; Ph.D.^&lowast ; ; Yasutaka Shuno ; M.D.^&lowast ; ; Kazuhito Sasaki ; M.D.^&lowast ; ; Manabu Kaneko ; M.D.^&lowast ; ; Masaya Hiyoshi ; M.D.^&lowast ; ; Eiji Sunami ; M.D. ; Ph.D.^&lowast ; ; Joji Kitayama ; M.D. ; Ph.D.^&lowast ; ; Koki Takahashi ; M.D. ; Ph.D.^&dagger ; ; Hirokazu Nagawa ; M.D. ; Ph.D.^&lowast ;
• 关键词：CD133 ; colon cancer cell ; chemoresistance ; integrin
• 刊名：Journal of Surgical Research
• 出版年：2012
• 期刊代码：299_00224804
• 类别：med
• 出版时间：15 June, 2012
• 卷：175
• 期：2
• 页码：278-288
• 文件大小：760 K

摘要

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Background and Aim

Recently, the cancer stem cells (CSCs) theory has been proposed, and CD133 has been suggested as a potential marker of CSCs in various cancer types. In the present study, we aimed evaluate CD133 as a potential marker of colorectal CSCs and, for this purpose, isolated CD133(+) and CD133(鈭? cells from a single colorectal cancer cell line, and compared their features, especially related to the tumor-forming and differentiation abilities, and the sensitivity to chemotherapy.

Methods and Results

CD133(+) cells had higher in聽vivo tumor-forming ability than CD133(鈭? cells, and in culture, they progressively differentiated into CD133(鈭? cells, but not vice-versa. On the other hand, CD133(鈭? cells were more resistant to 5-fluorouracil (FU) treatment than CD133(+) cells, and it was found to be dependent on the higher expression of 脽1-integrins, and consequently, higher ability to bind collagen. Disruption of the 脽1-integrin function abrogated the chemoresistance.

Conclusion

From the present results, we concluded that colorectal cancer CD133(+) cells, although showing some features of CSCs, are not more resistant to 5-FU than CD133(鈭? cells. Therefore, definite conclusions can not be drawn yet, but it is strongly suggestive that CD133 should not be used as a single CSC marker of colorectal cancer.