TLR-induced NF-魏B activation regulates NLRP3 expression in murine macrophages

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• 作者：Yu Qiao ; Peng Wang ; Jianni Qi ; Lei Zhang ; Chengjiang Gao ; ^{cgao@sdu.edu.cn}
• 关键词：TLR ; toll-like receptor ; NLRP3 ; NOD-LRRs containing pyrin domain 3 ; NF-魏B ; nuclear factor kappa B ; CHIP ; chromatin immunoprecipitation ; EMSA ; electrophoretic mobility shift assay ; nt ; nucleotide
• 刊名：FEBS Letters
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：5 April, 2012
• 卷：586
• 期：7
• 页码：1022-1026
• 文件大小：530 K

摘要

NLRP3 inflammasome plays a critical role in the activation of caspase-1 and maturation of IL-1尾. However, the specific cis- and trans-regulatory elements that determine the extent of NLRP3 expression are not well defined. In this study, we found NLRP3 expression was induced by TLR agonists in murine macrophages in a NF-魏B dependent manner. Furthermore, the corresponding NF-魏B binding sites (nt 鈭?303 to 鈭?292 and 鈭?238 to 鈭?228) were identified in the NLRP3 promoter. Finally, EMSA and ChIP assays demonstrated LPS-induced NF-魏B binding to the NLRP3 promoter. Therefore, out results delineated the molecular mechanisms involved in TLR-induced transcriptional regulation of NLRP3.