The effects of ouabain on resting membrane potential and hyperpolarization-activated current in neonatal rat nodose ganglion neurons

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• 作者：Shigeji Matsumoto ; Junichi Kitagawa ; Mamoru Takeda
• 关键词：Ouabain ; Na⁺-K⁺ pump ; Resting membrane potential ; Hyperpolarization-activated current ; Nodose ganglion
• 刊名：Neuroscience Letters
• 出版年：2008
• 期刊代码：52_03043940
• 类别：neu
• 出版时间：18 July 2008
• 卷：439
• 期：3
• 页码：241-244
• 文件大小：283 K

摘要

To determine whether the responses of resting membrane potential (RMP) and hyperpolarization-activated current (I_H) are altered by the application of ouabain, one of the Na⁺-K⁺ pump inhibitors, in neonatal rat small-diameter (<30 μm) nodose ganglion (NG) neurons, we examined the effects of 1 μM ouabain on those responses using perforated patch-clamp techniques. In current-clamp mode, the RMP was 40.2 ± 1.6 mV (n = 31). Twenty of 31 cells tested were depolarized by ouabain application, and these responses were associated with an increase in the cell input resistance. In the remaining 11 cells studied, 3 showed hyperpolarization in response to ouabain and 8 showed no effect on RMP. In voltage-clamp mode, 1 μM ouabain application enhanced the I_H in all of 10 neurons examined. These results suggest that ouabain application at 1 μM is capable of setting both the RMP level and the neuronal excitability in small-diameter NG neurons.