Cardioprotection of Asperosaponin X on experimental myocardial ischemia injury

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• 作者：Wang-Lin Jiang^a ; ^b ; Shu-Ping Zhang^a ; ^{davidjiangwl@163.com} ; Hai-Bo Zhu^b ; Jian Hou^b
• 关键词：Asperosaponin X ; Myocardial ischemia and reperfusion ; High mobility group box-1 protein ; Nuclear factor kappaB ; Inflammatory response
• 刊名：International Journal of Cardiology
• 出版年：2012
• 期刊代码：128_01675273
• 类别：med
• 出版时间：22 March, 2012
• 卷：155
• 期：3
• 页码：430-436
• 文件大小：1259 K

摘要

Background

Asperosaponin X was isolated from the roots of Dipsacus asper. In this study, we investigated the anti-myocardial ischemia and reperfusion (I/R) injury effects of Asperosaponin X in vivo and elucidated the potential mechanism in vitro.

Results

Asperosaponin X significantly attenuated hypoxia-induced cytotoxicity in a concentration-dependent manner in H9c2 cells. Treatment of H9c2 cells with Asperosaponin X 5 渭M or 10 渭M blocked TNF-伪-induced nuclear factor kappaB (NF-魏B) phosphorylation by blocking HMGB1 expression. Treatment of rats with Asperosaponin X 10 mg/kg, (i.v.) protected the animals from myocardial I/R injury as indicated by a decrease in infarct volume, improvement in hemodynamics and reduction of myocardial damage severity. Treatment with Asperosaponin X also lowered serum levels of pro-inflammatory factors and reduced High mobility group box-1 protein (HMGB1), phosphorylated I魏B-伪 and NF-魏B expression in ischemic myocardial tissue. Additionally, continuous i.v. of Asperosaponin X 14 days attenuated cardiac remodeling.

Conclusions

These protective effects suggested that Asperosaponin X may be due to block of myocardial inflammatory cascades through an HMGB1-dependent NF-魏B signaling pathway.