• 作者：P. Montenegro Á ; lvarez De Tejera^a ; ^{pmonal@oc.mde.es} ; C. Gutié ; rrez Ortega^a ; J. Gó ; mez De Terreros^b ; M.A. Cabanes Mariscal^a ; P. Sá ; nchez Ló ; pez^a ; M. Hijosa Pedregosa^a
• 关键词：Enfermedad pulmonar obstructiva cr&oacute ; nica ; Catabolismo muscular ; Pseudouridina ; Creatinina ; Cromatografí ; a lí ; quida de alta resoluci&oacute ; n ; Curva ROC ; Punto de corte
• 刊名：Revista de Patolog铆a Respiratoria
• 出版年：2011
• 期刊代码：pca325_15769895
• 类别：med
• 出版时间：January-March 2011
• 卷：14
• 期：1
• 页码：4-9
• 文件大小：217 K

Introduction

Chronic obstructive pulmonary disease (COPD) is the fourth leading cause of death in the world, appreciating muscle involvement, asymptomatic in early stages, which is due to a protein imbalance that has been measured in other processes by the urinary excretion of pseudouridine.

Objectives

To research, using urinary excretion of pseudouridine, if muscle catabolism in COPD is high and until what extent is related to age and gender.

Material and methods

The study is based on an analysis of a prospective randomized control group and one with COPD. Simultaneous determination of pseudouridine and creatinine in urine was performed by high-performance liquid chromatography (HPLC). We performed a descriptive statistical study of the variables age, gender, excretion of pseudouridine, and a ROC curve of the variable pseudouridine excretion, determines the cut point with higher sensitivity and specificity between the two populations.

Results

No statistically significant differences were found in the pseudouridine excreted, with respect to age or gender, in the groups studied. Pseudouridine excretion was significantly higher in patients with COPD, 94.40 (87.64) 渭mol/mmol, with respect to the control group, 19.90 (6.6) 渭mol/mmol Md (IQR) (p < 0.001). In the ROC curves analysis, pseudouridine excretion had area under the curve 0.972 and the optime cut point for pseudouridine excretion in our trial was 29.39 渭mol/mmol (Sensibility 93.1%and Specificity 100%) and can be used to distinguish people with altered muscle catabolism.

Conclusions

The measure of pseudouridine excreted in urine, performed by this method, can be used as a control of muscle breakdown. In our study, the cut point was 29.39 渭mol/mmol which means, that people with a pseudouridine excretion 29.39 渭mol/mmol, shall be individuals with an increased catabolism due to COPD.