The Varicella-zoster virus ORF54 gene product encodes the capsid portal protein, pORF54

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• 作者：Alexander J. Howard^a ; Debra M. Sherman^b ; Melissa A. Visalli^a ; Denise M. Burnside^a ; ¹ ; Robert J. Visalli^a ; ^{visallir@ipfw.edu}
• 关键词：Varicella-zoster virus ; ORF54 ; pORF54 ; Encapsidation ; Portal
• 刊名：Virus Research
• 出版年：2012
• 期刊代码：92_01681702
• 类别：imm
• 出版时间：July, 2012
• 卷：167
• 期：1
• 页码：102-105
• 文件大小：683 K

摘要

The Varicella-zoster virus (VZV) ORF54 gene was characterized using a guinea pig antiserum prepared to a GST-pORF54 fusion protein. A protein of the predicted size, 87 kDa, was detected in VZV-infected MeWo cells but not in mock-infected cells. Sucrose density gradient fractionation of pORF54 expressed in a recombinant baculovirus system resulted in samples containing enriched amounts of pORF54. Electron microscopic analysis suggested that the ORF54 gene encodes a protein that assembles into ring-like portal structures similar to those observed for numerous bacteriophages and other herpesviruses.