RNA self-ligation: From oligonucleotides to full length ribozymes

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• 作者：Slawomir Gwiazda ^{slawomir.gwiazda@rub.de} ; Kristina Salomon ^{kristina.salomon@uni-greifswald.de} ; Bettina Appel ^{bappel@uni-greifswald.de} ; Sabine Mü ; ller ; ^{smueller@uni-greifswald.de}
• 关键词：Cyclic phosphate ; RNA ligation ; RNA world ; Self-organization ; Self-replication
• 刊名：Biochimie
• 出版年：2012
• 期刊代码：24_03009084
• 类别：bio
• 出版时间：July, 2012
• 卷：94
• 期：7
• 页码：1457-1463
• 文件大小：539 K

摘要

The RNA-world-theory is one possible explanation of how life on earth has evolved. In this context it is of high interest to search for molecular systems, capable of self-organization into structures with increasing complexity. We have engineered a simple catalytic system in which two short RNA molecules can catalyze their own ligation to form a larger RNA construct. The system is based on the hairpin ribozyme using a 2鈥?3鈥?cyclophosphate as activated species for ligation. 2鈥?3鈥?cyclic phosphates can be easily formed and occur in many natural systems, thus being superior candidates for activated building blocks in RNA world scenarios.