Salvianolic acid A protects human SH-SY5Y neuroblastoma cells against H₂O₂-induced injury by increasing stress tolerance ability

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• 作者：Heng-ai Zhang ; Mei Gao ; Li Zhang ; Yan Zhao¹ ; Li-li Shi² ; Bai-nian Chen³ ; Yue-hua Wang ; Shou-bao Wang ; Guan-hua Du ; ^{dugh@imm.ac.cn}
• 关键词：Sal A ; Salvianolic acid A ; H2O2 ; hydrogen peroxide ; ATP ; adenosine triphosphate ; AMPK ; AMP-activated protein kinase ; rCBF ; regional cerebral blood flow ; PI3K ; phosphoinositide 3-kinase ; ROS ; reactive oxygen species ; PKB ; protein kinase B ; DMEM ; Dulbecco&r
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：11 May, 2012
• 卷：421
• 期：3
• 页码：479-483
• 文件大小：471 K

摘要

Salvianolic acid A (Sal A) is a polyphenol extracted from the root of the Salvia miltiorrhiza bunge. Hydrogen peroxide (H₂O₂) is a major reactive oxygen species (ROS), which has been implicated in stroke and other neurodegenerative diseases, including Alzheimer鈥檚 disease and Parkinson鈥檚 disease. In this study, we investigated the neuroprotective effects of Sal A in human SH-SY5Y neuroblastoma cells against H₂O₂-induced injury. Our results showed that cells pretreated with Sal A exhibited enhanced neuronal survival and that this protection was associated with an increase in adenosine triphosphate (ATP) and the stabilization of mitochondrial membrane potential. In addition, Sal A markedly decreased the excessive activation AMP-activated protein kinase (AMPK) and the serine-threonine protein kinase, Akt, in SH-SY5Ycells induced by H₂O₂. In conclusion, our results demonstrated that Sal A protects SH-SY5Y cells against H₂O₂-induced oxidative stress and these protective effects are related to stress tolerance and not energy depletion via inhibition of the AMPK and Akt signaling pathway.