Indocyanine green-loaded biodegradable tumor targeting nanoprobes for in聽vitro and in聽vivo imaging

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• 作者：Cuifang Zheng^a ; Mingbin Zheng^a ; ^b ; Ping Gong^a ; Dongxue Jia^a ; Pengfei Zhang^a ; Bihua Shi^a ; Zonghai Sheng^a ; Yifan Ma^a ; Lintao Cai^a ; ^{lt.cai@siat.ac.cn}
• 关键词：Indocyanine green ; Near-infrared nanoprobe ; Tumor targeting ; Molecular imaging ; Polymeric nanoparticles
• 刊名：Biomaterials
• 出版年：2012
• 期刊代码：7_01429612
• 类别：ms
• 出版时间：August, 2012
• 卷：33
• 期：22
• 页码：5603-5609
• 文件大小：1255 K

摘要

Indocyanine green (ICG) is a near-infrared (NIR) 铿倁orescence dye for extensive biological application, but limited by its poor aqueous stability in聽vitro, concentration-dependent aggregation, rapid elimination from the body, and lack of target specificity. In this paper, to overcome these limitations, folate receptor-targeted, ICG dye-doped poly(d,l-lactide-co-glycolide) (PLGA) lipid nanoparticles (FA-ICG-PLGA-lipid NPs) were constructed by a single-step self-assemble and nanoprecipitation method. The prepared FA-ICG-PLGA-lipid NPs exhibited good biocompatibility, monodispersity, excellent NIR penetration ability, significant stability against photobleaching and long circulation time. The intracellular uptake experiment proved the targeting efficacy of the FA-ICG-PLGA-lipid NPs was more effective in folate receptor over-expressing MCF-7 cells than folate receptor negative A549 cells. Furthermore, the in聽vivo experiments showed the FA-ICG-PLGA-lipid NPs were specifically targeted to the tumor, and its circulation time was much longer than free ICG. These biocompatible and biodegradable NIR-NPs prove a potential application in tumor diagnosis and targeted imaging due to its high aqueous stability, excellent NIR optical properties and significantly targeting property in聽vivo.