Prostaglandin J₂ series induces the gene expression of monocyte chemoattractant protein-1 during the maturation phase of cultured adipocytes

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• 作者：Mohammad Salim Hossain^a ; ^c ; ^{pharmasalim@yahoo.com} ; Kohji Nishimura^b ; Mitsuo Jisaka^a ; Tsutomu Nagaya^a ; Kazushige Yokota^a ; ^{yokotaka@life.shimane-u.ac.jp}
• 关键词：PG ; prostaglandin ; COX ; cyclooxygenase ; 15d-PGJ2 ; 15-deoxy-螖12 ; 14-prostaglandin J2 ; PPAR纬 ; peroxisome proliferator-activated receptor 纬 ; MCP-1 ; monocyte chemoattractant protein-1 ; IL-6 ; interleukin-6 ; L-PGDS ; lipocalin-type PGD synthase ; DME-HEPES ; Dul
• 刊名：Gene
• 出版年：2012
• 期刊代码：73_03781119
• 类别：bio
• 出版时间：10 July, 2012
• 卷：502
• 期：2
• 页码：138-141
• 文件大小：376 K

摘要

Prostaglandin (PG) J₂ series including 螖¹²-PGJ₂ and 15-deoxy-螖^12,14-prostaglandin J₂ (15d-PGJ₂) is the dehydration products of PGD₂ that are biosynthesized through the cyclooxygenase (COX) pathway. These prostanoids are active ligands for peroxisome proliferator-activated receptor 纬 (PPAR纬), a master regulator of adipogenesis in adipocytes. Here we investigated whether PGJ₂ derivatives can modulate the gene expression of monocyte chemoattractant protein-1 (MCP-1), a pro-inflammatory chemokine, during the maturation phase of adipocytes. Each of selective or nonselective inhibitors for COX isoforms suppressed significantly the accumulation of fats by interfering the induced expression of the PPAR纬 gene. Immunological assays of PGJ₂ series revealed higher production of 螖¹²-PGJ₂ than 15d-PGJ₂ by cultured adipocytes, implicating the contribution of endogenous PGJ₂ series to the stimulated adipogenesis. In addition, the increased transcription of MCP-1 was detectable at later maturation phase of adipogenesis, which was prevented by co-incubation with aspirin. Although 15d-PGJ₂ was more potent than 螖¹²-PGJ₂, both PGJ₂ derivatives series had similar effects to rescue dose-dependently the expression of the MCP-1 gene attenuated by aspirin. These findings suggest that the expression of MCP-1 involved in adipocyte inflammation could be positively regulated by the PGJ₂ series during adipogenesis in adipose tissue.