Complete genome sequence of a novel picorna-like virus isolated from Spodoptera exigua

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• 作者：Jae Young Choi^a ; Yang-Su Kim^a ; Yong Wang^b ; Sang Woon Shin^c ; Iksoo Kim^d ; Xue Ying Tao^a ; Qin Liu^a ; Jong Yul Roh^e ; Jae Su Kim^f ; Yeon Ho Je^a ; ^{btrus@snu.ac.kr}
• 关键词：Picorna-like virus ; Iflavirus ; Spodoptera exigua ; Complete genome ; Gene organization ; Phylogenetic analysis
• 刊名：Journal of Asia-Pacific Entomology
• 出版年：2012
• 期刊代码：P5_12268615
• 类别：abs
• 出版时间：June, 2012
• 卷：15
• 期：2
• 页码：259-263
• 文件大小：943 K

摘要

The complete genome sequence and the gene organization of a novel insect picorna-like virus, Spodoptera exigua virus (SeV), were determined. The genomic RNA of the SeV was 9501 nt in length excluding the poly(A) tail and contained a single, large open reading frame (nt 392-9424) encoding a 3010 aa polyprotein. Sequence comparisons with other viral polyproteins revealed that the consensus sequences for picornavirus RNA helicase, cysteine protease, and RNA-dependent RNA polymerase (RdRp) proteins are found on the genome in that order from the 5鈥?to the 3鈥?end. In terms of sequence similarity, identity, and genome organization, SeV resembled insect picorna-like viruses belonging to the genus Iflavirus. A phylogenetic analysis based on the eight conserved domains in the RdRp sequence showed that SeV was most closely related to the Perina nuda virus and Ectropis obliqua picorna-like virus, suggesting that these three insect picorna-like viruses might share a common ancestor.