Cycle and duration of the seminiferous epithelium in puma (Puma concolor)
- 作者：Flaviana Lima Guiã ; o Leite ; Tarcí ; zio Antô ; nio Rego de Paula ; Sé ; rgio Luis Pinto da Matta ; Clá ; udio Cesar Fonseca ; Marco Tú ; lio David das Neves and Joã ; o Bosco Gonç ; alv
- 关键词：Puma concolor ; Spermatogenesis ; Cycle of seminiferous ephitelium
- 刊名：Animal Reproduction Science
- 出版年：2006
- 期刊代码：8_03784320
- 类别：abs
- 出版时间：February 2006
- 卷：91
- 期：3-4
- 页码：307-316
- 文件大小：246 K
摘要
Puma or sussuarana (Puma concolor) is the second largest feline in the American continent and has an ample latitudinal distribution in very diverse habitats. In relation to its conservation status, the puma is considered an extinction-threatened species. The study of the testis morphology and the spermatogenic process in a species is fundamental for establishing the physiologic patterns that will make possible the selection of the protocols for assisted reproduction. A number of peculiarities associated with the reproductive biology of specific species such as the duration of spermatogenic process can be used to determine the frequency of sperm collection. Nine adult male pumas maintained in captivity were used to determine the relative frequency of stages in the seminiferous epithelium cycle. Three of them received intra-testicular injections of 0.1 ml tritiated thymidine to determine the duration of the seminiferous epithelium cycle, and were subjected to biopsy 7 days later. The cycle of the seminiferous epithelium in puma was didactically described into eight stages by the tubular morphology method. The total duration of one seminiferous epithelium cycle in puma was calculated to be 9.89 days, and approximately 44.5 days are required for development of spermatozoon from spermatogonia. The duration of spermiogenesis, prophase and other events of meiosis were 14.08, 15.20 and 1.79 days, respectively. The relative frequency of the pre-meiotic, meiotic and post-meiotic phases were 3.98, 1.79 and 4.12 days, respectively.