Field confinement with aberration correction for solid immersion lens based fluorescence correlation spectroscopy
- 作者：Ramach ; ra Rao ; Jelena Mitic ; Alex ; re Serov ; Rainer A. Leitgeb ; Theo Lasser
- 关键词：Fluorescence correlation spectroscopy ; Solid immersion lens ; Aberration compensation
- 刊名：Optics Communications
- 出版年：2007
- 期刊代码：50_00304018
- 类别：ph
- 出版时间：15 March 2007
- 卷：271
- 期：2
- 页码：462-469
- 文件大小：619 K
摘要
The solid immersion lens (SIL) as a tool for increasing the field confinement as well as providing optimal performance by aberration compensation in a confocal fluorescence correlation spectroscopy (FCS) system is illustrated here. Using Zernike polynomials we show that aberration compensation and the resultant pre-shaping of the incident wavefront enables near diffraction-limited performance. This is explained based on vectorial computations for high apertures in the Debye approximation. The obtained axial resolution parameters are compared with the obtained diffusion times in a SIL-FCS experiment for measurements in solutions done at the single molecule level.