Heterologous prime-boost regimen adenovector 35-circumsporozoite protein vaccine/recombinant Bacillus Calmette-Gu茅rin expressing the Plasmodium falciparum circumsporozoite induces enhanced long-term memory immunity in BALB/c mice

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• 作者：Charles Arama^a ; ^{charles.arama@wgi.su.se} ; ^{charama@icermali.org} ; Yohannes Assefaw-Redda^a ; Ariane Rodriguez^b ; Carmen Ferná ; ndez^a ; Giampietro Corradin^c ; Stefan H.E. Kaufmann^d ; Stephen T. Reece^d ; Marita Troye-Blomberg^a ; ^{marita.troye-blomberg@wgi.su.se}
• 关键词：Malaria vaccines ; Plasmodium falciparum circumsporozoite protein ; Heterologous prime-boost ; Ad35-CS ; BCG-CS ; Long-lived plasma cells
• 出版年：2012
• 期刊代码：89_0264410x
• 类别：imm
• 出版时间：8 June, 2012
• 卷：30
• 期：27
• 页码：4040-4045
• 文件大小：280 K

摘要

Background

Sustained antibody levels are a hallmark of immunity against many pathogens, and induction of long-term durable antibody titers is an essential feature of effective vaccines. Heterologous prime-boost approaches with vectors are optimal strategies to improve a broad and prolonged immunogenicity of malaria vaccines.

Results

In this study, we demonstrate that the heterologous prime-boost regimen Ad35-CS/BCG-CS induces stronger immune responses by enhancing type 1 cellular producing-cells with high levels of CSp-specific IFN-纬 and cytophilic IgG2a antibodies as compared to a homologous BCG-CS and a heterologous BCG-CS/CSp prime-boost regimen. Moreover, the heterologous prime-boost regimen elicits the highest level of LLPC-mediated immune responses.

Conclusion

The increased IFN-纬-producing cell responses induced by the combination of Ad35-CS/BCG-CS and sustained type 1 antibody profile together with high levels of LLPCs may be essential for the development of long-term protective immunity against liver-stage parasites.