Recent advances on multidimensional liquid chromatography-mass spectrometry for proteomics: From qualitative to quantitative analysis鈥擜 review

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• 作者：Qi Wu^a ; ^b  ; [Author Vitae] ; Huiming Yuan^a  ; [Author Vitae] ; Lihua Zhang^a ; ^{lihuazhang@dicp.ac.cn}  ; [Author Vitae] ; Yukui Zhang^a  ; [Author Vitae]
• 关键词：Multidimensional liquid chromatography ; Qualitative analysis ; Quantitative analysis ; Proteomics
• 刊名：Analytica Chimica Acta
• 出版年：2012
• 期刊代码：2_00032670
• 类别：ch
• 出版时间：20 June, 2012
• 卷：731
• 期：Complete
• 页码：1-10
• 文件大小：908 K

摘要

With the acceleration of proteome research, increasing attention has been paid to multidimensional liquid chromatography-mass spectrometry (MDLC-MS) due to its high peak capacity and separation efficiency. Recently, many efforts have been put to improve MDLC-based strategies including 鈥渢op-down鈥?and 鈥渂ottom-up鈥?to enable highly sensitive qualitative and quantitative analysis of proteins, as well as accelerate the whole analytical procedure. Integrated platforms with combination of sample pretreatment, multidimensional separations and identification were also developed to achieve high throughput and sensitive detection of proteomes, facilitating highly accurate and reproducible quantification. This review summarized the recent advances of such techniques and their applications in qualitative and quantitative analysis of proteomes.