Using high resolution melting analysis to identify variation of NPY, LEP and IGF-1 genes in Angus cattle

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• 作者：A.I. Trujillo^a ; ^{anatrujister@gmail.com} ; F. Peñ ; agaricano^a ; M.P. Grignola^a ; P. Nicolini^b ; A. Casal^a ; A.C. Espasandí ; n^a ; H. Naya^a ; ^c ; M. Carriquiry^a ; P. Chilibroste^a
• 关键词：HRM ; SNPs ; NPY ; Leptin ; IGF-1 ; Angus breed
• 刊名：Livestock Science
• 出版年：2012
• 期刊代码：12_18711413
• 类别：abs
• 出版时间：July, 2012
• 卷：146
• 期：2-3
• 页码：193-198
• 文件大小：390 K

摘要

Neuropeptide Y (NPY), leptin (LEP) and insulin-like growth factor 1 (IGF-1) have been proposed as valuable predictors of relative differences among individuals in the regulation of whole-body energy metabolism. The main objective of this study was to assess the allelic frequency of relevant single nucleotide polymorphisms (SNPs) in NPY, LEP and IGF-1 genes using high resolution melting (HRM) analysis. One hundred thirty six female calves from five Aberdeen Angus stud farms were genotyped. An A/G SNP located in intron 2 of the NPY gene (SNP_NPY), a C/T SNP located in exon 2 of the LEP gene (SNP_LEP), and a T/C SNP located in the promoter region of IGF-1 gene (SNP_IGF-1), were the markers genotyped. The favorable allele frequencies in the whole population were 0.23, 0.48 and 0.39 for SNP_NPY, SNP_LEP and SNP_IGF-1, respectively. The average value of F_ST for the three SNPs in the whole population was 0.015. The analysis of these SNPs indicated a low frequency of favorable alleles and a high homogeneity of the Angus population evaluated. The HRM genotyping described in this report provides a convenient alternative approach to identify SNPs in bovine genes.