Biogenesis of Y RNA-derived small RNAs is independent of the microRNA pathway

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• 作者：Francisco Esteban Nicolas¹ ; ² ; Adam E. Hall² ; Tibor Csorba ; Carly Turnbull ; Tamas Dalmay ; ^{T.Dalmay@uea.ac.uk}
• 关键词：Y RNA ; Small non-coding RNA ; microRNA ; Dicer ; Ago2
• 刊名：FEBS Letters
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：24 April, 2012
• 卷：586
• 期：8
• 页码：1226-1230
• 文件大小：748 K

摘要

Y RNAs are approximately 100 nucleotide long conserved cytoplasmic non-coding RNAs, which produce smaller RNA fragments during apoptosis. Here we show that these smaller RNA molecules are also produced in non-stressed cells and in a range of human cancerous and non-cancerous cell types. Recent reports have speculated that the cleavage products of Y RNAs enter the microRNA pathway. We tested this hypothesis and found that Y5 and Y3 RNA fragments are Dicer independent, they are in different complexes than microRNAs and that they are not co-immunoprecipitated with Ago2. Therefore we conclude that Y RNA fragments do not enter the microRNA pathway.