Effects of ultraviolet light on human serum 25-hydroxyvitamin D and systemic immune function

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• 作者：Sarah V.I. Milliken ; BMedSci^a ; ^&lowast ; ; Heather Wassall ; PhD^a ; ^&lowast ; ; Barry J. Lewis ; PhD^a ; Judith Logie ; RGN^b ; Robert N. Barker ; PhD^a ; ^&Dagger ; ; Helen Macdonald ; PhD^a ; ^&Dagger ; ; Mark A. Vickers ; FRCPath^a ; ^&Dagger ; ; Anthony D. Ormerod ; FRCP^a ; ^&Dagger ; ; ^{a.d.ormerod@abdn.ac.uk}
• 关键词：Narrowband UV-B ; vitamin D ; regulatory T cells ; eczema ; psoriasis ; FoxP3
• 刊名：Journal of Allergy and Clinical Immunology
• 出版年：2012
• 期刊代码：158_00916749
• 类别：med
• 出版时间：June, 2012
• 卷：129
• 期：6
• 页码：1554-1561
• 文件大小：260 K

摘要

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Background

Many immune-mediated diseases are associated with low levels of vitamin D and sunlight. UV light or supplementation with vitamin D can increase regulatory T-cell activity and prevent animal models of autoimmune disease. Increasing population vitamin D levels may therefore alleviate the burden of human immune-mediated disease.

Objective

To determine the responses of circulating 25-hydroxyvitamin D [25(OH)D] levels, regulatory T-cell numbers, and immune function to UV light exposure in patients being treated for skin disease.

Methods

Twenty-four subjects with skin disease from the North of Scotland were recruited between December and March. At baseline, and after 2 and 4 weeks of narrowband UV light exposure, we measured peripheral blood 25(OH)D level, numbers of regulatory T cells (CD4⁺CD25^hiFoxP3⁺), and T-cell proliferative and cytokine responses to anti-CD3/CD28 stimulation.

Results

Median (interquartile range) narrowband UV-B received during the study was 39.1 (30.9) as standard erythema dose, comparable to a quarter of the median summer sunlight exposure received locally. This increased the 25(OH)D level from a mean 卤 SD of 34 卤 17 nmol/L to 58 卤 16 nmol/L after 2 weeks and 78 卤 19 nmol/L after 4 weeks. The mean proportion of circulating regulatory T cells increased from 0.5%to 1.6%CD3⁺ cells, which significantly correlated with the increased 25(OH)D level. UV treatment was also followed by reduced proliferative and IL-10 responses to anti-CD3/CD28 independent of the 25(OH)D level.

Conclusion

Narrowband UV light reduces systemic immune responsiveness via the induction of regulatory T cells. Light and 25(OH)D levels may affect particular immune functions independently. The levels of serum 25(OH)D over which these effects are apparent should guide future interventions.