Lobaplatin induces apoptosis and arrests cell cycle progression in human cholangiocarcinoma cell line RBE

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• 作者：Zhaohai Wang^a ; Xuelian Tang^c ; Yilu Zhang^d ; Ruizhao Qi^a ; Zhiwei Li^a ; Keming Zhang^a ; Zhenwen Liu^a ; ^{Liuzhenwen@medmail.com.cn} ; Xinying Yang^b ; ^{xinying-y@163.com}
• 关键词：Lobaplatin ; Cholangiocarcinoma ; Cell cycle ; Apoptosis
• 刊名：Biomedicine and Pharmacotherapy
• 出版年：2012
• 期刊代码：36_07533322
• 类别：med
• 出版时间：April, 2012
• 卷：66
• 期：3
• 页码：161-166
• 文件大小：1048 K

摘要

The aim of this study was to determine the anticancer effects of lobaplatin in cholangiocarcinoma (CCA) RBE cells. We also explored the mechanism of action of lobaplatin by analyzing its influence on apoptosis and cell cycle. Our findings have shown that lobaplatin inhibits cell proliferations in human CCA cells with an IC50 value of approximately 5.26 卤 0.63 渭g/mL. Flowcytometry assay confirmed that lobaplatin affected CCA cell survival by blocking cell cycle progression and inducing apoptosis. Lobaplatin treatment reduced Cyclin D1, CDK4, and CDK6 expression, which led to the blocking of G0/G1 transition. In addition, lobaplatin increased p53, Bax expression, caspase-3 and poly (ADP-ribose) polymerase (PARP) cleavage, and reduced Bcl-2 expression, which contributed to the apoptosis of CCA cells. Lobaplatin showed a good anti-tumour activity in in vitro models of human CCA cells. These results indicate that Lobaplatin, as the third-generation platinum antineoplastic agent, could be an effective chemotherapeutic agent in human CCA treatment through induction apoptosis and cell cycle arrest.