Drought-induced modifications of photosynthetic electron transport in intact leaves: Analysis and use of neural networks as a tool for a rapid non-invasive estimation

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• 作者：Vasilij Goltsev^a ; Ivelina Zaharieva^b ; Petko Chernev^b ; Margarita Kouzmanova^a ; Hazem M. Kalaji^c ; ^{hazem@kalaji.pl} ; Ivan Yordanov^a ; Vassilena Krasteva^a ; Vladimir Alexandrov^a ; Detelin Stefanov^a ; Suleyman I. Allakhverdiev^d ; ^e ; Reto J. Strasser^f
• 关键词：ANN ; artificial neural network ; DF ; delayed fluorescence ; FM and F0 ; maximum and minimum fluorescence intensity during the induction period ; J and I ; intermediate steps in the chlorophyll a fluorescence rise (OJIP) appearing between F0 and FM at about 3 a
• 刊名：Biochimica et Biophysica Acta (BBA)/Bioenergetics
• 出版年：2012
• 期刊代码：13_00052728
• 类别：bio
• 出版时间：August, 2012
• 卷：1817
• 期：8
• 页码：1490-1498
• 文件大小：1113 K

摘要

Water deficit is one of the most important environmental factors limiting sustainable crop yields and it requires a reliable tool for fast and precise quantification. In this work we use simultaneously recorded signals of photoinduced prompt fluorescence (PF) and delayed fluorescence (DF) as well as modulated reflection (MR) of light at 820 nm for analysis of the changes in the photosynthetic activity in detached bean leaves during drying. Depending on the severity of the water deficit we identify different changes in the primary photosynthetic processes. When the relative water content (RWC) is decreased to 60%there is a parallel decrease in the ratio between the rate of excitation trapping in the Photosystem (PS) II reaction center and the rate of reoxidation of reduced PSII acceptors. A further decrease of RWC to 20%suppresses the electron transfer from the reduced plastoquinone pool to the PSI reaction center. At RWC below values 15%, the reoxidation of the photoreduced primary quinone acceptor of PSII, Q_A^-, is inhibited and at less than 5%, the primary photochemical reactions in PSI and II are inactivated. Using the collected sets of PF, DF and MR signals, we construct and train an artificial neural network, capable of recognizing the RWC in a series of 鈥渦nknown鈥?samples with a correlation between calculated and gravimetrically determined RWC values of about R² 鈮?#xA0;0.98. Our results demonstrate that this is a reliable method for determination of RWC in detached leaves and after further development it could be used for quantifying of drought stress of crop plants in situ. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.