Cytokine and CXC chemokine expression patterns in aqueous humor of patients with presumed tuberculous uveitis

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• 作者：Ahmed M. Abu El-Asrar^a ; ^{abuasrar@KSU.edu.sa} ; ^{abuelasrar@yahoo.com} ; Sofie Struyf^b ; Dustan Kangave^a ; Saleh A. Al-Obeidan^a ; Ghislain Opdenakker^b ; Karel Geboes^c ; Jo Van Damme^b
• 关键词：Tuberculosis ; Uveitis ; Cytokines ; Chemokines
• 刊名：Cytokine
• 出版年：2012
• 期刊代码：97_10434666
• 类别：imm
• 出版时间：August, 2012
• 卷：59
• 期：2
• 页码：377-381
• 文件大小：195 K

摘要

Aqueous humor (AH) samples from 14 patients with presumed tuberculous uveitis (PTU), and 30 control patients were assayed for the proinflammatory cytokines interleukin IL-4, IL-12, IL-15, IL-17, interferon (IFN)-纬, and tumor necrosis factor (TNF)-伪, the immunosuppressive cytokine IL-10, and the chemokines GRO-伪/CXCL1, IL-8/CXCL8, MIG/CXCL9, IP-10/CXCL10 and SDF-1/CXCL12 with the use of a multiplex assay. Among cytokines, IL-4 and IL-12 were not detected. IL-15, IL-17, IFN-纬, TNF-伪 and IL-10 levels in AH were significantly higher in patients than in controls (p < 0.001; p = 0.004; p < 0.001; p < 0.001; p < 0.001, respectively). Among chemokines, SDF-1 levels did not differ significantly between patients and controls, whereas GRO-伪, IL-8, MIG and IP-10 levels were significantly higher in patients than in controls (p = 0.001; p < 0.001; p < 0.001; p < 0.001, respectively). Mean GRO-伪 levels in AH of PTU patients were 6-fold higher than IL-8 levels and mean IP-10 levels were 15-fold higher than MIG levels. Clinical disease activity correlated significantly with the levels of IL-15, IFN-纬, TNF-伪 and IP-10. Logistic regression analysis demonstrated a significant positive association between PTU and high levels of IFN-纬, IL-8, MIG and IP-10. These data suggest that both T helper (Th) Th₁ and Th₁₇ cells are involved in PTU and that the cytokine profile is polarized toward a Th₁ response. GRO-伪 and IP-10 might be involved in neutrophil and activated T lymphocyte chemoattraction in PTU, respectively.