- 作者：Tina Histing ; M.D.&lowast ; ; &Dagger ; ; tina.histing@uks.eu ; David Stenger&lowast ; ; &Dagger ; ; Swantje Kuntz&lowast ; ; &Dagger ; ; Claudia Scheuer ; M.D.&dagger ; ; &Dagger ; ; Andrea Tami ; Dr sc techn§ ; ; Patric Garcia ; M.D.&lowast ; ; &Dagger ; ; Joerg H. Holstein ; M.D.&lowast ; ; &Dagger ; ; Moritz Klein ; M.D.&lowast ; ; &Dagger ; ; Tim Pohlemann ; M.D.&lowast ; ; &Dagger ; ; Michael D. Menger ; M.D.&dagger ; ; &Dagger ;
- 关键词：osteoporosis ; fracture healing ; SAMP6 mice ; osteoblasts ; osteoclasts ; gender ; remodeling ; osteocalcin (OC) ; tartrate-resistent acid phosphatase (TRAP) ; deoxypyridinoline (DPD)
- 刊名：Journal of Surgical Research
- 出版年：2012
- 期刊代码：299_00224804
- 类别：med
- 出版时间：15 June, 2012
- 卷：175
- 期：2
- 页码：271-277
- 文件大小：182 K
ass="h4">Background
Previous studies have shown that fracture healing depends on gender and that in females, ovariectomy-induced osteoporosis impairs the healing process. There is no information, however, whether the alteration of fracture healing in osteoporosis also depends on gender.ass="h4">Materials and Methods
Therefore, we herein studied fracture healing in female and male senescence-accelerated osteoporotic mice, strain P6 (SAMP6), including biomechanical, histomorphometric, and protein biochemical analysis.
ass="h4">Results
Bending stiffness was reduced in male and female SAMP6 mice compared with senescence-resistant strain 1 (SAMR1) controls. This was associated with elevated serum concentrations of tartrate-resistent acid phosphatase form 5b (TRAP) in both female and male SAMP6 mice. Callus size, however, was significantly larger in female SAMP6 mice compared with male SAMP6 mice and female SAMR1 controls. This indicates a delayed remodeling process in female SAMP6 mice. The delay of callus remodeling in female SAMP6 mice was associated with a significantly higher osteoprotegerin (OPG) callus tissue expression and increased serum concentrations of osteocalcin (OC) and deoxypyridinoline (DPD), indicating elevated osteoblast and osteoclast activities.
ass="h4">Conclusion
The present study shows that remodeling during fracture healing in female, but not in male, SAMP6 mice is delayed, most probably due to an increased osteoblast and osteoclast activity.