Preparation and characterisation of poly(high internal phase emulsion) methacrylate monoliths and their application as separation media
- 作者：Krajnc ; Peter ; Leber ; Nermina ; &Scaron ; tefanec ; Dejan ; Kontrec ; Sandra ; Podgornik ; Ale&scaron
- 关键词：Methacrylate monoliths ; PolyHIPE ; Emulsion polymerisation ; Protein separation ; Polymer chromatographic supports
- 刊名：Journal of Chromatography A
- 出版年：2005
- 期刊代码：47_00219673
- 类别：ch
- 出版时间：February 11, 2005
- 卷：1065
- 期：1
- 页码：69-73
- 文件大小：311 K
摘要
Poly(glycidyl methacrylate-co-ethyleneglycol dimethacrylate) monolithic supports were prepared by radical polymerisation of the continuous phase of water in oil high internal phase emulsions. Morphology of monolithic materials was studied by scanning electron microscopy and mercury intrusion porosimetry. The ratio of phase volume and the degree of crosslinking influenced the void size and pore size distribution of resulting polymers. Void sizes between 1 and 10μm were observed and average pore sizes around 100nm. Polymers with 60, 75, 80 and 90%pore volume were prepared and even samples with highest pore volume showed good mechanical stability. They were modified to bear weak-anion exchange groups and tested on the separation of standard protein mixture containing myoglobin, conalbumine and trypsin inhibitor. Good separation was obtained in a very short time similar to the separation obtained by commercial methacrylate monoliths. However, higher dispersion was observed. Bovine serum albumin dynamic binding capacity for monolith with 90%porosity was close to 9mg/ml.