Comparative evaluation of the activity of antivirals towards feline immunodeficiency virus in different cell culture systems

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• 作者：F.J.U.M. van der Meer ; N.M.P. Schuurman ; J. Balzarini ; H.F. Egberink
• 关键词：Antiviral testing ; NRTI ; Plant lectins ; FIV ; In vitro assay
• 刊名：Antiviral Research
• 出版年：2007
• 期刊代码：7_01663542
• 类别：imm
• 出版时间：November 2007
• 卷：76
• 期：2
• 页码：198-201
• 文件大小：137 K

摘要

Influences of the cell system on observed EC₅₀ values of different agents against feline immunodeficiency virus (FIV) were assessed. The activity of various nucleoside reverse transcriptase inhibitors (NRTI) against a lymphotropic FIV strain was evaluated using monocultured thymocytes and a DC–thymocyte coculture. In the second set of experiments activity of carbohydrate binding agents (CBA) towards FIV strains derived from different cell lines (e.g. Crandall feline kidney cells (CRFK) and thymocytes) was compared. We examined three different FIV-based antiviral evaluation systems and obtained marked differences in EC₅₀ values, especially for CBA entry inhibitors. Our study confirms and extends earlier observed differences between cell systems used for the evaluation of the activity of antivirals towards FIV.