Inhibition of adhesion of Clostridium difficile to Caco-2 cells

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• 作者：Naaber ; Paul ; Lehto ; Elina ; Salminen ; Seppo ; Mikelsaar ; Marika
• 关键词：Clostridium difficile ; Colostrum ; Xylitol ; Mucosal adhesion ; Caco-2 cell
• 刊名：FEMS Immunology and Medical Microbiology
• 出版年：1996
• 期刊代码：32_09288244
• 类别：imm
• 出版时间：July, 1996
• 卷：14
• 期：4
• 页码：205-209
• 文件大小：379 K

摘要

For many microorganisms, including Clostridium difficile, mucosal association is an important factor influencing intestinal colonisation and subsequent infection. Inhibition of adhesion of C. difficile to intestinal mucosa could be a new promising strategy for prevention and treatment of antibiotic-associated diarrhoea. We investigated the possibilities of influencing the adhesion of C. difficile by xylitol and bovine colostrum whey. Caco-2 cells and C. difficile cells were incubated with 1%, 5%and 10%solutions of xylitol and colostrum. Our study revealed that both xylitol and colostrum inhibited the adhesion of C. difficile to Caco-2 cells. Inhibition by xylitol was dose-dependent. When compared to the control, the count of adherent C. difficile decreased 3.4 times when treated with 1%xylitol, 12 times when 5%xylitol was applied, and 18.7 times when treated with 10%xylitol. The inhibition of adherence by colostrum was partially dose-dependent: 3.1 times in the case of 1%, and 5.5 times in the cases of 5%and 10%colostrum. Further experimental and clinical studies are needed for the application of xylitol and colostrum in the treatment and prophylaxis of pseudomembraneous colitis.