Ginsenoside Rb₁ promotes adipogenesis in 3T3-L1 cells by enhancing PPARγ₂ and C/EBPα gene expression

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• 作者：Wenbin Shang ; Ying Yang ; Boren Jiang ; Hua Jin ; Libin Zhou ; Shangquan Liu ; Mingdao Chen
• 关键词：Ginsenosides Rb₁ ; Adipogenesis ; 3T3-L1 adipocytes ; PPARγ ; C/EBPα ; Proliferation ; GLUT1 ; GLUT4
• 刊名：Life Sciences
• 出版年：2007
• 期刊代码：62_00243205
• 类别：imm
• 出版时间：23 January 2007
• 卷：80
• 期：7
• 页码：618-625
• 文件大小：606 K

摘要

Evidence has accumulated that ginseng and its main active constituents, ginsenosides, possess anti-diabetic and insulin-sensitizing properties which may be partly realized by regulating adipocyte development and functions. In the present study, we explored the effect of ginsenoside Rb₁, the most abundant ginsenoside in ginseng root, on adipogenesis of 3T3-L1 cells. We found that with standard differentiation inducers, ginsenoside Rb₁ facilitated adipogenesis of 3T3-L1 preadipocytes in a dose-dependent manner; 10 μM Rb₁ increased lipid accumulation by about 56%. Treatment of differentiating adipocytes with 10 μM Rb₁ increased the expression of mRNA and protein of PPARγ₂ and C/EBPα, as well as mRNA of ap2, one of their target genes. After the treatment of differentiating adipocytes with Rb₁, basal and insulin-mediated glucose uptake was significantly augmented, accompanied by the up-regulation of mRNA and protein level of GLUT4, but not of GLUT1. In addition, ginsenoside Rb₁ also inhibited the proliferation of preconfluent 3T3-L1 preadipocytes. Our data indicate that anti-diabetic and insulin-sensitizing activities of ginsenosides, at least in part, are involved in the enhancing effect on PPARγ2 and C/EBPα expression, hence promoting adipogenesis.