CXCR4 engagement promotes dendritic cell survival and maturation
- 作者：Kenji Kabashima ; Kazunari Sugita ; Noriko Shiraishi ; Hirokazu Tamamura ; Nobutaka Fujii ; Yoshiki Tokura
- 关键词：Langerhans cell ; Dendritic cell ; Maturation ; Survival ; Antagonist ; CXCR4 ; CXCL12 ; Proliferation
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：5 October 2007
- 卷：361
- 期：4
- 页码：1012-1016
- 文件大小：293 K
摘要
It has been reported that human monocyte derived-dendritic cells (DCs) express CXCR4, responsible for chemotaxis to CXCL12. However, it remains unknown whether CXCR4 is involved in other functions of DCs. Initially, we found that CXCR4 was expressed on bone marrow-derived DCs (BMDCs). The addition of specific CXCR4 antagonist, 4-F-Benzoyl-TN14003, to the culture of mouse BMDCs decreased their number, especially the mature subset of them. The similar effect was found on the number of Langerhans cells (LCs) but not keratinocytes among epidermal cell suspensions. Since LCs are incapable of proliferating in vitro, these results indicate that CXCR4 engagement is important for not only maturation but also survival of DCs. Consistently, the dinitrobenzene sulfonic acid-induced, antigen-specific in vitro proliferation of previously sensitized lymph node cells was enhanced by CXCL12, and suppressed by CXCR4 antagonist. These findings suggest that CXCL12–CXCR4 engagement enhances DC maturation and survival to initiate acquired immune response.