Everolimus quantification in peripheral blood mononuclear cells using ultra high performance liquid chromatography tandem mass spectrometry

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• 作者：Francois Roullet-Renoleau^a ; Florian Lemaitre^a ; Marie Antignac^b ; Noel Zahr^c ; Robert Farinotti^a ; Christine Fernandez^a ; ^b ; ^{christine.fernandez@sat.aphp.fr}
• 关键词：Everolimus ; Liquid chromatography tandem mass spectrometry ; Peripheral blood mononuclear cells ; Therapeutic drug monitoring
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2012
• 期刊代码：65_07317085
• 类别：ch
• 出版时间：July, 2012
• 卷：66
• 期：Complete
• 页码：278-281
• 文件大小：258 K

摘要

A reliable ultra high performance liquid chromatography tandem mass spectrometry method has been developed for the determination of everolimus in human peripheral blood mononuclear cells (PBMCs). Protein precipitation was used for sample preparation. Analysis was performed on an UPLC Waters Acquity system. Chromatography was carried out using a cartridge column MassTrak TDM C18 (2.1 脳 10 mm) with 0.1%formic acid and 2 mM of ammonium acetate in water and 0.1%formic acid and 2 mM of ammonium acetate in methanol mixture as a mobile phase delivered at a flow rate of 0.4 mL/min in gradient mode. The assay was linear over a range of 0-12.5 ng/mL. The analysis of quality control samples at 2.5, 5.0 and 10.0 ng/mL demonstrated good precision with relative standard deviation of less than 15%. Recoveries at concentrations of 2.5, 5.0 and 10.0 ng/mL were all greater than 83%. The method was successfully applied to the analysis of everolimus in PBMCs from blood samples of transplant recipients.