Evaluation of a low cost cryopreservation system on the biology of human amniotic fluid-derived mesenchymal stromal cells

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• 作者：Jose Maria Miranda-Sayago^a ; ^b ; ^{josem.miranda.exts@juntadeandalucia.es} ; ^{txetxu@uma.es} ; Nieves Fernandez-Arcas^a ; ^b ; Carmen Benito^c ; Armando Reyes-Engel^b ; Jose Ramon Herrero^d ; Antonio Alonso^a ; ^b
• 关键词：hAMSC ; amniotic fluid-derived stromal cells ; hMSC ; human mesenchymal stromal cells ; DMSO ; dimethyl sulfoxide ; PD ; population doublings
• 刊名：Cryobiology
• 出版年：2012
• 期刊代码：166_00112240
• 类别：bio
• 出版时间：June, 2012
• 卷：64
• 期：3
• 页码：160-166
• 文件大小：647 K

摘要

Background

Human amniotic-derived mesenchymal stromal cells (hAMSC) are a novel population of multipotent stem cells that have been shown to have great potential for use in regenerative medicine. However, procedures to store and preserve hAMSC for future clinical applications have not been explored extensively.

Methods

In this study, we analyzed the influence of cryopreservation, using a protocol based on freezing rate of 1 掳C/min, 10%dimethyl sulfoxide as cryoprotectant and a thawing rate >100 掳C/min, on hAMSC morphology, proliferation rates, viability, cell cycle, karyotype, immune phenotype and multilineage differentiation potential.

Results

This study found that this cryopreservation protocol does not affect the biological properties of hAMSC.

Discussion

This shows that this protocol is a viable system for banking hAMSC, with the associated advantages that has a low cost in terms of expense, time and personnel involved and is easy to implement.