Sugarcane leaves: Pretreatment and ethanol fermentation by Saccharomyces cerevisiae

详细信息	查看全文 | 推荐本文 |

• 作者：Rumpa Jutakanoke^a ; Natchanun Leepipatpiboon^b ; Vasana Tolieng^c ; Vichien Kitpreechavanich^d ; Teerapatr Srinorakutara^e ; Ancharida Akaracharanya^a ; ^{sanchari@chula.ac.th}
• 关键词：Sugarcane leaves ; Ethanol ; Pretreatment ; Lignocellulose ; Saccharomyces cerevisiae
• 刊名：Biomass and Bioenergy
• 出版年：2012
• 期刊代码：3_09619534
• 类别：pw
• 出版时间：April, 2012
• 卷：39
• 期：Complete
• 页码：283-289
• 文件大小：492 K

摘要

The susceptibility of sugarcane leaves to cellulase hydrolysis by GC220 cellulase (10 filter paper units (FPU)/gram dry weight basis (g, DS)) for 72聽h was compared after pretreatment by autoclaving at 121聽掳C, 15 pounds per square inch (lb/in²) in the presence of either dilute sulfuric acid or lime. The optimal (from those evaluated) conditions for each type of pretreatment of green sugarcane leaves (20-40 mesh particle) at 6%weight by volume (w/v, DS), were found to be 1.5%(w/v) sulfuric acid and 2%(w/v) calcium hydroxide for 30 and 15聽min, respectively. The acid pretreated samples released more glucose than the lime treated ones (5.7%and 3.9%weight by weight (w/w, DS), respectively). Accellerase鈩?1000 hydrolysis (160聽FPU/g, DS) of the dilute sulfuric acid pretreated ground sugarcane leaves suspended in 0.5聽M citrate buffer, or in pretreatment hydrolysate yielded 0.104 and 0.163聽g glucose/g (DS) of leaves after 6聽h, respectively. Fermentation of the two above obtained glucose sources by Saccharomyces cerevisiae for 12 and 24聽h, respectively, yielded ethanol at 4.8%and 8.0%(w/w, DS), respectively.