Fractionation of wheat straw by prehydrolysis, organosolv delignification and enzymatic hydrolysis for production of sugars and lignin

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• 作者：W.J.J. Huijgen ; ^{huijgen@ecn.nl} ; A.T. Smit ; P.J. de Wild ; H. den Uil
• 关键词：Organosolv ; Pretreatment ; Enzymatic hydrolysis ; Prehydrolysis ; Biorefining
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：June, 2012
• 卷：114
• 期：Complete
• 页码：389-398
• 文件大小：1150 K

摘要

Wheat straw was fractionated using a three-step biorefining approach: (1) aqueous pretreatment for hemicellulose prehydrolysis into sugars, (2) organosolv delignification, and (3) enzymatic cellulose hydrolysis into glucose. Prehydrolysis was applied to avoid degradation of hemicellulose sugars during organosolv delignification. Maximum xylose yield obtained was 67%or 0.17 kg/kg straw (prehydrolysis: 175 掳C, 30 min, 20 mM H₂SO₄) compared to 4%in case of organosolv without prehydrolysis (organosolv: 200 掳C, 60 min, 60%w/w aqueous ethanol). Prehydrolysis was found to reduce the lignin yield by organosolv delignification due to the formation of 鈥榩seudo-lignin鈥?and lignin recondensation during prehydrolysis. This reduction could partly be compensated by increasing the temperature of the organosolv delignification step. Prehydrolysis substantially improved the enzymatic cellulose digestibility from 49%after organosolv without prehydrolysis to 80%(20 FPU/g substrate). Increasing the organosolv delignification temperature to 220 掳C resulted in a maximum enzymatic glucose yield of 93%or 0.36 kg/kg straw.