Effective determination method for a cyanobacterial neurotoxin, β-N-methylamino-l-alanine

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• 作者：Takuya Kubo ; Noriko Kato ; Ken Hosoya ; Kunimitsu Kaya
• 关键词：Cyanobacteria ; Neurotoxin ; BMAA ; LC– ; ESI-MS ; HILIC ; SIM
• 刊名：Toxicon
• 出版年：2008
• 期刊代码：50_00410101
• 类别：pha
• 出版时间：1 June 2008
• 卷：51
• 期：7
• 页码：1264-1268
• 文件大小：226 K

摘要

We developed a simple and effective analysis procedure that includes pretreatment and determination methods for β-N-methylamino-l-alanine (BMAA), a cyanobacterial neurotoxin. BMAA may be produced by all known groups of cyanobacteria living in freshwater as well as marine environments. In this paper, we report a novel determination method for BMAA. A cation-exchange resin was effective for the selective concentration of BMAA from cyanobacterial extracts and yielded a high recovery rate. Moreover, liquid chromatography (LC)–electrospray ionization mass spectrometry with a hydrophilic LC column was effective for determining BMAA levels. The quantitation limit for BMAA based on selected ion monitoring (SIM) was determined as 0.5 ng at a signal/noise ratio of 5.