Increase of chondrogenic potentials in adipose-derived stromal cells by co-delivery of type I and type II TGF尾 receptors encoding bicistronic vector system

详细信息	查看全文 | 推荐本文 |

• 作者：Sun-Woong Kang^a ; Hyun-Jin Do^a ; In-Bo Han^b ; Dong-Ah Shin^b ; Hyun Ok Kim^c ; Jae-Hwan Kim^a ; ^{jaehwan_k@cha.ac.kr} ; Soo-Hong Lee^a ; ^{soohong@cha.ac.kr}
• 关键词：Adipose derived stromal cells ; Transforming growth factor 尾 ; Type I TGF尾 receptor ; Type II TGF尾 receptor ; Gene delivery ; Chondrogenic differentiation
• 刊名：Journal of Controlled Release
• 出版年：2012
• 期刊代码：25_01683659
• 类别：pha
• 出版时间：28 June, 2012
• 卷：160
• 期：3
• 页码：577-582
• 文件大小：885 K

摘要

Stem cell therapy has been developing rapidly as a potential cure for repairing or regenerating the functions of diseased organs and tissues. Adipose-derived stromal cells (ASCs) are an attractive cell source for stem cell therapy because they can be isolated easily from fat tissue in significant numbers and exhibit multiple differentiation potential under appropriate in vitro culture conditions. However, ASCs derived from individual donors can show wide variations in differentiation potential. In addition, the regulatory mechanisms underlying stem cell differentiation remain unclear. Transforming growth factor 尾 (TGF尾) is a well-known ASC chondrogenic differentiation factor that stimulates ASC signaling pathways by activating transmembrane type I and type II receptors. We hypothesized that the chondrogenic differentiation potential of ASCs is dependent upon the expression of TGF尾 receptors and could be improved by the co-delivery of type I (TGF尾RI) and type II (TGF尾RII) TGF尾 receptors. To prove this, heterogeneity within the chondrogenic potential of ASCs isolated from 10 donors was examined and their susceptibility to TGF尾 during the process of chondrogenic differentiation investigated. In addition, the results showed that co-delivery of the TGF尾RI and TGF尾RII genes increased the expression of TGF尾 receptor signaling in ASCs with low chondrogenic potential, resulting in increased chondrogenic differentiation. Monitoring and delivering TGF尾RI and TGF尾RII may, therefore, be a powerful tool for predicting the differentiation potential of stem cells and for enhancing their differentiation capacity prior to stem cell transplantation.