MicroRNA-337 is associated with chondrogenesis through regulating TGFBR2 expression

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• 作者：N. Zhong^&dagger ; ; ^&Dagger ; ; ^a ; ^{zhongnan0911@126.com} ; J. Sun^&dagger ; ; ^&Dagger ; ; ^a ; Z. Min^&dagger ; ; ^&Dagger ; ; W. Zhao^&dagger ; ; ^&Dagger ; ; R. Zhang^&dagger ; ; ^&Dagger ; ; W. Wang^&dagger ; ; ^&Dagger ; ; J. Tian^&dagger ; ; ^&Dagger ; ; L. Tian^&dagger ; ; ^&Dagger ; ; J. Ma^&dagger ; ; ^&Dagger ; ; D. Li^&dagger ; ; ^&Dagger ; ; Y. Han^&dagger ; ; ^&Dagger ; ; S. Lu^&dagger ; ; ^&Dagger ; ; ^§ ; ; ^{lushemin@mail.xjtu.edu.cn} ; ^{shemin.lu@gmail.com}
• 关键词：miR-337 ; TGFBR2 ; Chondrogenesis
• 刊名：Osteoarthritis and Cartilage
• 出版年：2012
• 期刊代码：249_10634584
• 类别：med
• 出版时间：June, 2012
• 卷：20
• 期：6
• 页码：593-602
• 文件大小：1346 K

摘要

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Summary

Objective

MicroRNAs (miRNAs) have been implicated in regulating diverse cellular pathways and involved in development and inflammation. This study aimed to examine six miRNAs expression during the cartilage development and identify the key miRNA which is associated with chondrogenesis.

Methods

The expression of six miRNAs in cartilage tissue during development was screened by real-time quantitative polymerase chain reaction (RT-qPCR). Rat models of bone matrix gelatin induced endochondral ossification, collagen-induced arthritis and pristane-induced arthritis were established to examine whether miR-337 is involved in chondrogenesis. Furthermore, the regulation of transforming growth factor-b type II receptor (TGFBR2) expression by miR-337 was determined with the luciferase reporter gene assay and Western blot. The expression of some specific genes relevant to cartilage tissue was tested by RT-qPCR after miR-337 mimic or inhibitor transfection.

Results

MiR-337 expression was significantly down-regulated and almost disappeared in the maturation phases of endochondral ossification. The results of histology and RT-qPCR from three rat models showed that miR-337 is directly bound up with chondrogenesis. Furthermore, the results from the luciferase reporter gene assay and Western blot indicated that miR-337 regulated TGFBR2 expression. Our study also found that the enhancement of miR-337 may modulate the expression of cartilage-specific genes such as AGC1 in C-28/I2 chondrocytes.

Conclusion

We proved that miRNA-337 is associated with chondrogenesis through regulating TGFBR2 expression, and miRNA-337 can also influence cartilage-specific gene expression in chondrocytes. These findings may provide an important clue for further research in the arthritis pathogenesis and suggest a new remedy for arthritis treatment.