Transforming growth factor-β-induced gene product, as a novel ligand of integrin αMβ2, promotes monocytes adhesion, migration and chemotaxis
- 作者：Ha-Jeong Kim ; In-San Kim
- 关键词：β ; ig-h3/TGFBIp ; Monocyte ; Chemotaxis ; Adhesion ; Inflammation
- 刊名：The International Journal of Biochemistry and Cell Biology
- 出版年：2008
- 期刊代码：127_13572725
- 类别：med
- 出版时间：2008
- 卷：40
- 期：5
- 页码：991-1004
- 文件大小：791 K
摘要
Monocyte recruitment from the blood in response to chemoattractant gradients is a key phenomenon in inflammation. Various extracellular matrix proteins, at the site of inflammation, have chemoattractant activity and mediate monocyte adhesion and migration as ligands of integrins. In this report, we demonstrate that transforming growth factor-β-induced gene product (βig-h3/TGFBIp), as an extracellular matrix protein, mediates monocytes adhesion under both static and flow conditions mainly through integrin 
Mβ2. Fasciclin 1 domains of βig-h3/TGFBIp are responsible for the interaction with integrin Mβ2, not only enhances monocyte migration in both chemotactic and haptotactic manners but also mediates their transendothelial migration and subendothelial matrix invasion. These activities are also mediated through integrin Mβ2. Intraperitoneal injection of βig-h3/TGFBIp promotes the recruitment of monocytes but not neutrophils. Our results demonstrate that βig-h3/TGFBIp produced at inflammatory sites is a novel chemoattractant for monocytes and interacts with integrin Mβ2 to serve as a substrate for their migration, suggesting that βig-h3/TGFBIp plays an important role in inflammation.
Mβ2. Fasciclin 1 domains of βig-h3/TGFBIp are responsible for the interaction with integrin Mβ2, not only enhances monocyte migration in both chemotactic and haptotactic manners but also mediates their transendothelial migration and subendothelial matrix invasion. These activities are also mediated through integrin Mβ2. Intraperitoneal injection of βig-h3/TGFBIp promotes the recruitment of monocytes but not neutrophils. Our results demonstrate that βig-h3/TGFBIp produced at inflammatory sites is a novel chemoattractant for monocytes and interacts with integrin Mβ2 to serve as a substrate for their migration, suggesting that βig-h3/TGFBIp plays an important role in inflammation.