Protection of riboflavin and UVB sensitized degradation of DNA and RNA bases by natural antioxidants

详细信息	查看全文 | 推荐本文 |

• 作者：Prakash C. Joshi ; Thomas A. Gray ; Thomas C. Keane ; ^{keanet@sage.edu}
• 关键词：DNA and RNA bases ; Natural antioxidants ; Photosensitization ; Riboflavin ; UVB (290&ndash ; 320  ; nm)
• 刊名：Ecotoxicology and Environmental Safety
• 出版年：2012
• 期刊代码：77_01476513
• 类别：env
• 出版时间：1 April, 2012
• 卷：78
• 期：Complete
• 页码：86-90
• 文件大小：183 K

摘要

Riboflavin (RF) is a potent photosensitizer producing extensive degradation of purine and pyrimidine derivatives of nucleic acids under UVA, UVB and sunlight. In this study we have demonstrated that reactive O₂ species generated by photosensitized RF under UVB were responsible for the degradation of DNA and RNA bases. While ¹O₂ accounted for the degradation of adenine, guanine, thymine and uracil, O₂^{鈭捖?/sup>also contributed to partial degradation of adenine. Cytosine remained unaffected by the synergistic action of RF and UVB. Ascorbic acid, glutathione, glycolic acid and quercetin showed remarkable protection (88-100%) against photodegradation of bases. Sorbitol was effective in preventing photodegradation of guanine. These naturally occurring antioxidants are potential candidates for prevention against oxidative stress caused by photosensitization.}