• 作者：Jordi Reina^a ; ^{jorge.reina@ssib.es} ; Virginia Plasencia^a ; Maria Leyes^b ; Antonio Nicolau^c ; Antonia Galmé ; s^c ; Gabriel Arbona^c
• 关键词：Virus gripales ; Reacció ; n en cadena de la polimerasa en transcripció ; n reversa en tiempo real ; Enzimoinmunoaná ; lisis ; Cultivo shell-vial ; Adultos
• 刊名：Enfermedades Infecciosas y Microbiolog铆a Cl铆nica
• 出版年：2010
• 期刊代码：pca153_0213005X
• 类别：med
• 出版时间：February 2010
• 卷：28
• 期：2
• 页码：95-98
• 文件大小：170 K

Introduction

The age of the patients and the type of sample are major problems in the diagnosis of influenza. Most available diagnostic techniques are highly effective in pediatric patients and in nasopharyngeal aspirates. However, in the adult population and using throat swabs, these techniques are much less reliable.

Aim

We performed a prospective study comparing the efficacy of a commercial real-time reverse transcription PCR assay (RT-PCR) with that of an enzyme immunoassay (EIA) or shell vial culture (SV) in the detection of influenza A and B viruses in 125 throat swabs from adults with clinically suspected influenza during the 2007鈥?008 flu season.

Material and methods

Throat swabs were subjected to rapid antigen detection for influenza viruses by means of a commercial dot-blot EIA. For the RT-PCR technique, RNA was extracted from 200 渭L of each sample by the automated extraction system, EZ1 virus minikit (version 2.0). Genomic amplification of the extracted viral RNA was carried out using the OneStep RT-PCR FluA+FluB automated system with the SmartCycler amplification system. Each sample was inoculated into 2 SV of the MDCK cell line. Turnaround times were calculated from the time specimens were received in the laboratory to the time the result was reported to clinicians.

Results

The EIA system detected 27 (21.6%) positive samples, RT-PCR 62 (49.6%) positive samples, and SV 56 (44.8%) positive samples. Among the 62 positive samples, EIA detected 27 (43.5%), RT-PCR 62 (100%) and SV 56 (90.3%). With the use of RT-PCR, 38.4%of the adults studied were diagnosed on the same day samples were received. Among the total, 67.2%of diagnostic results were obtained within the first 24 hours; turnaround time was 1.1 days.

Conclusion

The real-time RT-PCR method studied displayed high sensitivity and specificity in the detection of influenza virus in adult patients, when compared with the conventional techniques. With real-time RT-PCR, large numbers of samples can be rapidly tested and results provided the same day samples are received.