Expression and distribution of fugu TIMP-2s (fgTIMP-2a and fgTIMP-2b) mRNAs in tissues and embryos
- 作者：Hiroshi Tsukamoto ; Yoshihiro Yokoyama ; Tohru Suzuki ; Shoshi Mizuta ; Reiji Yoshinaka
- 关键词：Embryo ; Fugu ; Quantitative real-time PCR ; Takifugu rubripes ; Teleost ; Tissue inhibitor of metalloproteinase (TIMP)-2a ; TIMP-2b ; Whole mount in situ hybridization
- 刊名：Comparative Biochemistry and Physiology ; Part B
- 出版年：2007
- 期刊代码：50_10964959
- 类别：bio
- 出版时间：November 2007
- 卷：148
- 期：3
- 页码：225-230
- 文件大小：462 K
摘要
In teleosts, two distinct types of TIMP-2s occur, TIMP-2a and TIMP-2b, but little is known about their locations and quantitative expressions. Here, we examined pufferfish (Takifugu rubripes) TIMP-2a (fgTIMP-2a) and TIMP-2b (fgTIMP-2b) quantities and locations in fugu adult tissues and embryos. To compare the quantitative expression of fgTIMP-2s, we performed a quantitative real-time PCR (qPCR). FgTIMP-2a mRNA was constitutively expressed and significant differences in expression were not observed among adult tissues. Whereas, fgTIMP-2b mRNA was significantly differently expressed in ordinary muscle and gill compared to the expression level in whole blood (P < 0.05). Although significant difference was not observed between brain and other tissues, both fgTIMP-2s mRNAs were abundant in the brain. In addition, we examined embryos during development using qPCR. Both fgTIMP-2s mRNAs gradually increased during embryonic development from 48 hpf. However, fgTIMP-2b mRNA was obviously abundant compared to fgTIMP-2a mRNA in embryos. We also examined the specific mRNA distribution in embryos. The fgTIMP-2s mRNAs showed the same distribution during development. Both fgTIMP-2s are expressed in adult fugu tissues and embryos but their expression levels clearly differ, suggesting that there is a predominance of fgTIMP-2b over fgTIMP-2a in vivo.