Seizure activity in dogs is associated with enhanced TIMP-2 expression of microglia

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• 作者：Veronika M Stein^a ; ^{veronika.stein@tiho-hannover.de} ; Sem Genini^b ; ^{geninis@vet.upenn.edu} ; Christina Puff^c ; ^{christina.puff@tiho-hannover.de} ; Wolfgang Baumgä ; rtner^c ; ^d ; ^{wolfgang.baumgaertner@tiho-hannover.de} ; Andrea Tipold^a ; ^d ; ^{andrea.tipold@tiho-hannover.de}
• 关键词：Microglia ; Epilepsy ; MMP ; TIMP
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：2012
• 期刊代码：105_01652427
• 类别：abs
• 出版时间：15 April, 2012
• 卷：146
• 期：2
• 页码：101-105
• 文件大小：221 K

摘要

In the pathogenesis of epilepsy aberrant synaptic plasticity plays an important role. Matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) are responsible for nervous tissue remodelling resulting in synaptic plasticity in the central nervous system (CNS) and might therefore be crucially involved in epileptogenesis. To assess the potential pathogenetic role of microglial MMPs and TIMPs in seizure induction, twenty-four dogs suffering from different intracranial diseases with and without seizure activity were comparatively examined. Microglial cells were isolated by density gradient centrifugation and their expression profiles of MMP-2, MMP-9, MMP-12, MMP-13, MMP-14, TIMP-1, TIMP-2, and RECK (reversion-inducing cysteine-rich protein with Kazal motifs) were examined via quantitative real-time PCR (qPCR). Interestingly, a significant up-regulation of TIMP-2 expression was found for the first time in dogs suffering from seizures. In conclusion, microglial TIMP expression might be involved in seizure generation.