Cartilage repair in transplanted scaffold-free chondrocyte sheets using a minipig model

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• 作者：Goro Ebihara^a ; Masato Sato^a ; ^{sato-m@is.icc.u-tokai.ac.jp} ; Masayuki Yamato^b ; Genya Mitani^a ; Toshiharu Kutsuna^a ; Toshihiro Nagai^a ; Satoshi Ito^a ; Taku Ukai^a ; Miyuki Kobayashi^a ; Mami Kokubo^a ; Teruo Okano^b ; Joji Mochida^a
• 关键词：Cartilage tissue engineering ; Chondrocyte ; Cell culture ; Animal model ; Transplantation
• 刊名：Biomaterials
• 出版年：2012
• 期刊代码：7_01429612
• 类别：ms
• 出版时间：May, 2012
• 卷：33
• 期：15
• 页码：3846-3851
• 文件大小：664 K

摘要

Lacking a blood supply and having a low cellular density, articular cartilage has a minimal ability for self-repair. Therefore, wide-ranging cartilage damage rarely resolves spontaneously. Cartilage damage is typically treated by chondrocyte transplantation, mosaicplasty or microfracture. Recent advances in tissue engineering have prompted research on techniques to repair articular cartilage damage using a variety of transplanted cells. We studied the repair and regeneration of cartilage damage using layered chondrocyte sheets prepared on a temperature-responsive culture dish. We previously reported achieving robust tissue repair when covering only the surface layer with layered chondrocyte sheets when researching partial-thickness defects in the articular cartilage of domestic rabbits. The present study was an experiment on the repair and regeneration of articular cartilage in a minipig model of full-thickness defects. Good safranin-O staining and integration with surrounding tissues was achieved in animals transplanted with layered chondrocyte sheets. However, tissue having poor safranin-O staining鈥攏ot noted in the domestic rabbit experiments鈥攚as identified in some of the animals, and the subchondral bone was poorly repaired in these. Thus, although layered chondrocyte sheets facilitate articular cartilage repair, further investigations into appropriate animal models and culture and transplant conditions are required.