Expression profiles of a MhCTR1 gene in relation to banana fruit ripening

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• 作者：Huei-Lin Hu^a ; Yi-Yin Do^a ; ^{yiyindo@ntu.edu.tw} ; Pung-Ling Huang^a ; ^b ; ^{pungling@ntu.edu.tw}
• 关键词：CTR1 ; Ethylene signaling ; Fruit ripening ; Climacteric fruit ; Musa spp.
• 刊名：Plant Physiology and Biochemistry
• 出版年：2012
• 期刊代码：87_09819428
• 类别：abs
• 出版时间：July, 2012
• 卷：56
• 期：Complete
• 页码：47-55
• 文件大小：1648 K

摘要

The banana (Musa spp.) is a typical climacteric fruit of high economic importance. The development of bananas from maturing to ripening is characterized by increased ethylene production accompanied by a respiration burst. To elucidate the signal transduction pathway involved in the ethylene regulation of banana ripening, a gene homologous to Arabidopsis CTR1 (constitutive triple response 1) was isolated from Musa spp. (Hsien Jin Chiao, AAA group) and designated as MhCTR1. MhCTR1 spans 11.5聽kilobases and consists of 15 exons and 14 introns with consensus GT-AG nucleotides situated at their boundaries. MhCTR1 encodes a polypeptide of 805 amino acid residues with a calculated molecular weight of 88.6聽kDa. The deduced amino acid sequence of MhCTR1 demonstrates 55%, 56%and 55%homology to AtCTR1, RhCTR1, and LeCTR1, respectively. MhCTR1 is expressed mostly in the mature green pulp and root organs. During fruit development MhCTR1 expression increases just before ethylene production rises. Moreover, MhCTR1 expression was detected mainly in the pulps at ripening stage 3, and correlated with the onset of peel yellowing, while MhCTR1 was constitutively expressed in the peels. MhCTR1 expression could be induced by ethylene treatment (0.01聽渭L聽L^鈭?), and MhCTR1 expression decreased in both peel and pulp 24聽h after treatment. Overall, changes observed in MhCTR1 expression in the pulp closely related to the regulation of the banana ripening process.