Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris
- 作者：Nobumitsu Sasaki ; chaki@cc.tuat.ac.jp ; Tatsuro Odawara ; Shoko Nagai ; Kazuma Yoshimura ; Yasuhiko Matsushita ; Hiroshi Nyunoya
- 关键词：Cell-to-cell movement ; Tomato mosaic virus ; Movement protein ; Transcriptional coactivator ; Transgenic tobacco ; Plasmodesmal targeting ; Plant protection
- 刊名：Physiological and Molecular Plant Pathology
- 出版年：2012
- 期刊代码：183_08855765
- 类别：bio
- 出版时间：April, 2012
- 卷：78
- 期：Complete
- 页码：38-44
- 文件大小：653 K
摘要
A putative transcriptional coactivator KELP of Arabidopsis thaliana and its homolog of Brassica campestris (BcKELP) can bind the movement protein (MP) of Tomato mosaic virus (ToMV) in聽vitro and, when transiently over-expressed, interfere with the cell-to-cell movement of ToMV. In this study, we generated and selected transgenic Nicotiana tabacum lines expressing BcKELP constitutively and examined their resistance to ToMV. We also investigated changes in MP localization in initially infected Nicotiana benthamiana cells expressing BcKELP transiently. Our results indicate that a high expression of BcKELP can reduce viral initial and short-distance movement probably through disturbance of plasmodesmal targeting of MP.