Genetic modulation in Be-78 and Y Trypanosoma cruzi strains after long-term infection in Beagle dogs revealed by molecular markers

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• 作者：Vanja Maria Veloso^c ; ^e ; ^{vanjaveloso@ef.ufop.br} ; Paulo Marcos da Matta Guedes^g ; Marta de Lana^a ; ^d ; Helen Rodrigues Martins^f ; Clá ; udia Martins Carneiro^a ; ^d ; Antô ; nia Clá ; udia Já ; come da Câ ; mara^g ; Daniella Alchaar D&rsquo ; Á ; vila^e ; Ivo Santana Caldas^a ; Lú ; cia Maria da Cunha Galvã ; o^e ; Egler Chiari^e ; Maria Terezinha Bahia^a ; ^b
• 关键词：Trypanosoma cruzi ; Chagas disease ; Beagle dogs ; Genetic modulation
• 刊名：Infection ; Genetics and Evolution
• 出版年：2012
• 期刊代码：119_15671348
• 类别：med
• 出版时间：July, 2012
• 卷：12
• 期：5
• 页码：1128-1135
• 文件大小：538 K

摘要

The genetic profile of Trypanosoma cruzi was evaluated in parasite populations isolated from Beagle dogs experimentally infected with Be-78 and Y strains that present distinct biological and genetic characteristics. Molecular characterization of the isolates obtained 30 days and 2 years after infection was carried out. For typing MLEE, sequence polymorphisms of the mitochondrial cytochrome oxidase subunit II gene (COII) and RAPD profiles were used. The profiles of MLEE were the same for the parental Be-78 strains as their respective isolates. However, changes of MLEE profile were observed in two T. cruzi isolates from dogs inoculated with Y strain. Changes in the mitochondrial DNA (COII) and RAPD profiles of the Y strain were also observed. The dendogram constructed by UPGMA with RAPD results indicated two major branches. Global data show that the genetic modulation in polyclonal strains during the long-term infection occurred and was strain-dependent. This study still suggests that each host (here each dog) harbors a determinate T. cruzi population that may change or be modulated throughout long-term infection. This might to hinder the observation of correlation between the genetics of T. cruzi and their biological properties and behavior in different host species due to the complexity of the parasite-host interaction in which probably the genetic background of both should be considered.