- 作者：L. Meseguer-Olmo<sup>asup><sup> ; sup><sup>lmeseguer.doc@gmail.comsup><sup> ; sup><sup>docmurcia@yahoo.comsup> ; A. Bernabeu-Escaplez<sup>asup> ; M. Vallet-Regí ; <sup>bsup> ; S. Aznar-Cervantes<sup>asup> ; V. Vicente-Ortega<sup>asup> ; M. Alcaraz-Bañ ; os<sup>csup> ; M. Clavel-Sainz<sup>asup> ; A. Herrera-Rodrí ; guez<sup>dsup> ; F. Lopez-Prats<sup>esup> ; J.M. Moraleda-Jimé ; nez<sup>asup> ; C.L. Meseguer-Ortiz<sup>asup>
- 关键词：Cerá ; mica ví ; trea ; Andamiaje ; Cé ; lulas madre mesenquimales adultas ; Osteoblastos ; Defecto ó ; seo
- 刊名：Revista Espa帽ola de Cirug铆a Ortop茅dica y Traumatolog铆a
- 出版年：2010
- 期刊代码：pca311_18884415
- 类别：med
- 出版时间：January-February 2010
- 卷：54
- 期：1
- 页码：59-68
- 文件大小：468 K
ss="h4">Purpose
To describe the development and characterization of a vitroceramic material as well as the initial response of adult mesenchymal stem (MSCs-A) isolated from bone marrow.
ss="h4">Material and methodology
The material was obtained by heating glass with composition in mol%55SiO<sub>2sub>-41CaO-4P<sub>2sub>O<sub>5sub> by a sol gel method. Cells were isolated from direct iliac crest aspirates from young adult patients. An analysis was performed of the degree of adhesion, proliferation and osteoblastic differentiation of MSCs-A seeded onto the material. Cell differentiation was evaluated through the production of osteocalcin and the loss of the CD90 mesenchymal marker. Cell proliferation on the substrate was performed using the tetrazolium salt reduction method. The seeded material was implanted in a critical defect caused in a rabbit femur in order to determine its osteogenerating capacity; CT observations were carried out.
ss="h4">Results
MSCs-A se bound to the material, expanded, proliferated and produced mineralized extracellular matrix on the material during the culture period. At the same time, they showed an osteoblastic phenotype, increasing osteocalcin production and losing CD90 expression. The material was partially resorbed at the end of the study.
ss="h4">Conclusion
The material is cytocompatible, osteoconductive, bioactive and has a capacity to promote osteoblastic differentiation of MSCs-A as well as new bone formation following its implantation in association with MSCs-A; an appropriate matrix for bone tissue regeneration.