Nicotine alters the ectonucleotidases activities in lymphocytes: In vitro and in vivo studies

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• 作者：Gustavo Roberto Thomé ; ^a ; Lizielle Souza de Oliveira^a ; Maria Rosa Chitolina Schetinger^a ; Vera Maria Morsch^a ; Rosé ; lia Maria Spanevello^d ; Amanda Maino Fiorenza^a ; Jonas Seres^a ; Jucimara Baldissarelli^a ; Naiara Stefanello^a ; Maria Ester Pereira^a ; Nicé ; ia Spanholi Calgaroto^a ; Victor Camera Pimentel^a ; Daniela Bitencourt Rosa Leal^e ; Viviane do Carmo Gonç ; alves Souza^e ; Jeandre Augusto dos Santos Jaques^a ; Claudio Alberto Martins Leal^a ; Ritiel Corrê ; a da Cruz^c ; Flá ; via Valladã ; o Thiesen^f ; Cinthia Melazzo Mazzanti^b ; ^{cmelazzomazzanti@gmail.com}
• 关键词：NTPDase ; Adenosine deaminase ; Nicotine ; Lymphocytes
• 刊名：Biomedicine and Pharmacotherapy
• 出版年：2012
• 期刊代码：36_07533322
• 类别：med
• 出版时间：April, 2012
• 卷：66
• 期：3
• 页码：206-212
• 文件大小：491 K

摘要

The aim of the present study was to investigate the effects in vivo and in vitro of nicotine, an important immunosuppressive agent, on NTPDase and ADA activities in lymphocytes of adult rats. The following nicotine doses in vivo study were evaluated: 0.0, 0.25 and 1.0 mg/kg/day injected subcutaneously in rats for 10 days. The activity of the enzymes were significantly decreased with nicotine 0.25 and 1 mg/kg which inhibited ATP (22%, 54%), ADP (44%, 30%) hydrolysis and adenosine (43%, 34%) deamination, respectively. The expression of the protein NTPDase in rat lymphocytes was decreased to nicotine 1 mg/kg and the lymphocytes count was decreased in both nicotine doses studied. The purine levels measured in serum of the rats treated with nicotine 0.25 mg/kg significantly increased to ATP (39%), ADP (39%) and adenosine (303%). The nicotine exposure marker was determinate by level of cotinine level which significantly increased in rats treated with nicotine 0.25 (39%) and 1 mg/kg (131%) when compared to rats that received only saline. The second set of study was in vitro assay which the ATP-ADP-adenosine hydrolysis were decreased by nicotine concentrations 1 mM (0%- 0%- 16%, respectively), 5 mM (42%- 32%- 74%, respectively), 10 mM (80%- 27%- 80%, respectively) and 50 mM (96%- 49%- 98%, respectively) when compared with the control group. We suggest that alterations in the activities of these enzymes may contribute to the understanding of the mechanisms involved in the suppression of immune response caused by nicotine.