Large-scale survey of porcine endogenous retrovirus in Chinese miniature pigs
- 作者：Jianmin Wu ; Yuyuan Ma ; Maomin Lv ; Yubiao Yang ; Yanru Guo ; Xiuling Yu ; Kegong Tian ; Jingang Zhang
- 关键词：Porcine endogenous retrovirus ; Chinese miniature pigs ; Xenotransplantation
- 刊名：Comparative Immunology ; Microbiology and Infectious Diseases
- 出版年：2008
- 期刊代码：52_01479571
- 类别：bio
- 出版时间：July 2008
- 卷：31
- 期：4
- 页码：367-371
- 文件大小：103 K
摘要
We conducted a large-scale survey on the existence and expression status of porcine endogenous retrovirus (PERV) in seven breeds of Chinese miniature pigs. Genotyping of PERV was examined by PCR using type-specific primers according to the env genotyping method. The presence and expression status of viral gag, pol and envgenes were further analyzed in Wuzhishan pigs (WZSP) and Bama minipigs (BMP). The results showed that PERV existed in all 348 genomic DNA samples. The genotype distribution was subtype A—74.43%, subtype B—95.40%and subtype C—30.46%. No expression of subtype C was found in WZSP and BMP. This research obtained an adequate level of information on the molecular epidemiology of PERV in China. The results indicated that it is possible to monitor pig herds for individuals with the lowest PERV prevalence, especially lacking PERV-C.