Differential expression of bla_SHV related to susceptibility to ampicillin in Klebsiella pneumoniae

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• 作者：Yingmei Fu ; Fengmin Zhang ; Wenli Zhang ; Xiaobei Chen ; Yuehui Zhao ; Jiayu Ma ; Lili Bao ; Wuqi Song ; Takeo Ohsugi ; Toru Urano ; et al.
• 关键词：bla gene ; Klebsiella pneumoniae ; Expression ; Reverse transcription-polymerase chain reaction
• 刊名：International Journal of Antimicrobial Agents
• 出版年：2007
• 期刊代码：126_09248579
• 类别：med
• 出版时间：March 2007
• 卷：29
• 期：3
• 页码：344-347
• 文件大小：132 K

摘要

Klebsiella pneumoniae usually shows intrinsic resistance to ampicillin and other β-lactams. bla_SHV is thought to be a key β-lactamase gene responsible for this intrinsic resistance to ampicillin. Nevertheless, surveys of clinical strains reveal that some isolates of K. pneumoniae that carry bla_SHV remain susceptible to ampicillin. To explore susceptibility to ampicillin in relation to bla_SHV in K. pneumoniae, we analysed the existence and transcription of bla_SHV and determined β-lactamase activity as well as the susceptibilities to clinically relevant β-lactams, including ampicillin in 160 K. pneumoniae isolates from China. In total, 141 isolates (88.1%) were detected as bla_SHV-positive, 20 (14.2%) of which were found to be broadly susceptible to all β-lactams tested, including ampicillin. Among the 20 broadly susceptible isolates, sequencing of bla_SHV revealed synonymous point mutations in 19 isolates and a premature stop codon in 1 isolate. Reverse transcription-polymerase chain reaction failed to detect bla_SHV mRNA in five isolates (25%). The results demonstrate that differential expression of bla_SHV in clinical isolates of K. pneumoniae can affect susceptibility to ampicillin.