A neutralizable epitope is induced on HGF upon its interaction with its receptor cMet

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• 作者：Kisu Kim ; Youngmi Hur ; En-Kyung Ryu ; Jung-Hyo Rhim ; Cha Yong Choi ; Cheol-Min Baek ; Jae-Ho Lee ; Junho Chung
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2007
• 期刊代码：159_0006291x
• 类别：bio
• 出版时间：2 March 2007
• 卷：354
• 期：1
• 页码：115-121
• 文件大小：616 K

摘要

A new conformational neutralizable epitope is created on heptocyte growth factor (HGF), when it interacts with its receptor, cMet. By immunizing rabbits with HGF–cMet complex, we successfully generated a monoclonal antibody (SFN68) that inhibits HGF–cMet interaction, and blocks the biological function mediated by HGF. To define the epitope, we screened out an epitope-mimicking peptide, KSLSRHDHIHHH, from a phage display of combinatorial peptide library. In molecular mimicry this peptide bound to cMet and inhibited HGF–cMet interaction. No humoral response was induced to this epitope-mimicking peptide when immunization was done with HGF alone.