Enantioselective metabolism and cytotoxicity of the chiral herbicide ethofumesate in rat and chicken hepatocytes

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• 作者：Xinyuan Xu¹ ; ^{xuxinyuan861130@gmail.com} ; Jinling Diao¹ ; ^{lingyinzi1201@gmail.com} ; Xinru Wang ^{514969974@qq.com} ; Ziheng Dang ^{zihengd@gmail.com} ; Ping Zhang ^{zhangpzxm@gmail.com} ; Yubo Li ^{465965102@qq.com} ; Zhiqiang Zhou ; ^{zzqcau@gmail.com}
• 关键词：Ethofumesate ; Stereoselective metabolism ; Stereoselective cytotoxicity ; Hepatocytes
• 刊名：Pesticide Biochemistry and Physiology
• 出版年：2012
• 期刊代码：182_00483575
• 类别：bio
• 出版时间：May, 2012
• 卷：103
• 期：1
• 页码：62-67
• 文件大小：694 K

摘要

We investigated the metabolic kinetics and toxicity of ethofumesate (ETO) in rat and chicken hepatocytes using a chiral high-performance liquid chromatographic (HPLC) method. The metabolic of ETO in rat hepatocytes was enantioselective, whereas it was not in chicken hepatocytes. The T_1/2 of (鈭?-ETO was about two times longer than that of (+)-ETO after the rat hepatocytes had been incubated with 20 渭M rac-ETO. There was no chiral conversion or transformation during their incubation with the hepatocytes. Toxicity differences were observed between the two enantiomers of ETO, reflected in their EC₅₀ values in rat and chicken hepatocytes. The stereoselective cytotoxicity of the two enantiomers was opposite in rat and chicken hepatocytes. We have developed a method of studying the toxicokinetics and cytotoxicity of chiral agrochemicals in hepatocytes isolated from mammals (rats) and chicken. The data presented here allow a more thorough understanding of this pesticide and should be useful in its full environmental assessment.