cGMP-dependent protein kinase I is involved in neurite outgrowth via a Rho effector, rhotekin, in Neuro2A neuroblastoma cells
- 作者：Keizo Yuasa ; yuasa@bio.tokushima-u.ac.jp ; Takeshi Nagame ; Makoto Dohi ; Yayoi Yanagita ; Shin Yamagami ; Masami Nagahama ; Akihiko Tsuji
- 关键词：NO ; nitric oxide ; cGK ; cGMP-dependent protein kinase ; GEF ; guanine nucleotide exchange factor ; GAP ; GTPase-activating protein ; 8-pCPT-cGMP ; 8-(4-chlorophenylthio)-cGMP ; RA ; all-trans-retinoic acid ; LPA ; 1-oleoyl lysophosphatidic acid ; VASP ; vasodilator-st
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2012
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：4 May, 2012
- 卷：421
- 期：2
- 页码：239-244
- 文件大小：746 K
摘要
Although the cGMP/cGMP-dependent protein kinase (cGK) signaling is involved in the regulation of neurite outgrowth, its mechanism remains to be clarified. In this study, we identified a Rho effector, rhotekin, as a cGK-I-interacting protein. Rhotekin was also a substrate for cGK-I伪. In neurite-extended Neuro2A neuroblastoma cells, cGK-I伪 and rhotekin were colocalized in the plasma membrane and extended neurites, while treatment with cGMP resulted in translocation of rhotekin to the cytoplasm. In addition, we found that cGK-I伪 and rhotekin synergistically suppressed Rho-induced neurite retraction. Our findings suggest that cGK-I伪 interacts with and phosphorylates rhotekin, thereby contributing to neurite outgrowth regulation.